Filter paper degrading ability of a Trichoderma strain with multinucleate conidia
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Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages
Published on: October 20, 2023
Hideo Toyama1, Makiko Yano, Takeshi Hotta
1Minamikyushu University, Kirishima 5-1-2, 880-0032, Miyazaki, Japan. wonder@iris.dti.ne.jp
Abstract:
The multinucleate conidia were produced from the green mature conidia of Trichoderma reesei Rut C-30 strain by colchicine treatment. The strain with higher Filter paper degrading ability was selected among those conidia using a double layer selection medium. The selected strain, JS-2 was able to collapse the filter paper within 15 min but the original strain took 25 min to collapse it completely. Moreover, the amount of reducing sugar in the L-type glass tube of the strain, JS-2 was greater than that of the original strain. The Avicel, CMC-Na, and Salicin hydrolyzing activity of the strain, JS-2, increased 2.1 times, 1.2 times, and 3.6 times higher than that of the original strain.

