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Related Experiment Video

Updated: Jan 2, 2026

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Single-cell microinjection technology in cell biology.

Yan Zhang1, Long-Chuan Yu

  • 1Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China. yanzhang@pku.edu.cn

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|May 15, 2008
PubMed
Summary
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Single-cell microinjection precisely delivers various molecules into difficult-to-transfect cells. This mechanical method offers controlled dosage and timing for diverse biological applications, reviewed herein.

Area of Science:

  • Cell biology
  • Molecular biology
  • Biotechnology

Background:

  • Single-cell microinjection is a technique for introducing substances into cells.
  • It is effective for delivering proteins, cDNA, peptides, drugs, and particles.
  • This method is valuable for cells resistant to conventional transfection.

Purpose of the Study:

  • To review the advantages and limitations of single-cell microinjection.
  • To discuss its applications in various biological research areas.
  • To highlight its utility for both attached and suspended cells.

Main Methods:

  • Microinjection as a mechanical delivery system.
  • Controlled dosage and precise timing of substance delivery.
  • Application to diverse cell types, including primary cultures.

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Last Updated: Jan 2, 2026

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Single-cell Microinjection for Cell Communication Analysis
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Main Results:

  • Successful delivery of exogenous molecules into transfection-challenged cells.
  • Wide applicability in transgenic animal production and in vitro fertilization.
  • Utility in RNA interference studies.

Conclusions:

  • Single-cell microinjection is a versatile and precise method for cellular delivery.
  • It overcomes limitations of other transfection techniques.
  • Its applications span fundamental research to biotechnology.