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Updated: Jul 5, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Characterization of human dental pulp-derived cell lines.

H Suguro¹, M Asano, Y Kaneko

¹Department of Endodontics, Dental Research Center, Nihon University School of Dentistry, Nihon Univeristy, Chiyoda-ku, Tokyo, Japan.

International Endodontic Journal

|May 16, 2008

Summary

Researchers established and characterized dental pulp fibroblastic cell lines. These cell lines exhibit distinct gene expression profiles, indicating the presence of unique subpopulations within dental pulp stem cells.

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Last Updated: Jul 5, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Area of Science:

Cell Biology
Stem Cell Research
Tissue Engineering

Background:

Dental pulp tissue is a source of mesenchymal stem cells.
Understanding dental pulp cell heterogeneity is crucial for regenerative medicine.

Purpose of the Study:

To establish and characterize novel fibroblastic cell lines from human dental pulp.
To investigate the heterogeneity of these dental pulp-derived cell lines.

Main Methods:

Human dental pulp cells were immortalized using SV40 large T antigen.
Cell lines were established via limiting dilution and geneticin selection.
Characterization involved immunofluorescence staining and differential display PCR.

Main Results:

Nine distinct fibroblastic cell lines were successfully established.
All cell lines expressed fibroblast markers (vimentin, collagen I & III).
Differential display revealed varied gene expression, including the T-cell marker Thy-1.

Conclusions:

Dental pulp-derived fibroblastic cell lines display heterogeneous gene expression profiles.
This heterogeneity suggests the existence of distinct subpopulations within dental pulp.
Findings contribute to understanding dental pulp stem cell diversity.