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Updated: Jul 5, 2026

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

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Published on: November 1, 2018

Glomerular endothelial cells form diaphragms during development and pathologic conditions.

Koichiro Ichimura¹, Radu V Stan, Hidetake Kurihara

¹Department of Anatomy, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan. ichimura@med.juntendo.ac.jp

Journal of the American Society of Nephrology : JASN

|May 16, 2008

Summary

Adult glomerular endothelial cells (GEnC) typically lack diaphragmed fenestrae. However, these structures and PV-1 protein reappear during kidney injury, suggesting a role in glomerular capillary remodeling.

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Published on: August 19, 2020

Area of Science:

Nephrology
Cell Biology
Histology

Background:

Adult glomerular endothelial cells (GEnC) are characterized by fenestrae, but the presence of diaphragms at these fenestrae remains debated.
Diaphragmed fenestrae are typically found in fenestrated capillary endothelial cells, playing a role in filtration.

Purpose of the Study:

To investigate the presence and characteristics of diaphragmed fenestrae in adult and embryonic rat GEnC.
To determine the expression of the transmembrane glycoprotein PV-1 in relation to diaphragmed fenestrae.
To explore the potential role of diaphragmed fenestrae and PV-1 in glomerular capillary remodeling following injury.

Main Methods:

Morphologic analysis of GEnC fenestrae using electron microscopy.
Immunocytochemical analysis to detect the expression of PV-1 protein.
Comparison of GEnC structure and PV-1 expression in embryonic and adult rats, including those with induced Thy-1.1 nephritis.

Main Results:

Adult rat GEnC predominantly lacked diaphragmed fenestrae containing PV-1, with only a small fraction exhibiting these structures.
Embryonic rat GEnC displayed diaphragmed fenestrae expressing PV-1 protein.
Adult rats with Thy-1.1 nephritis showed a significant increase in GEnC with diaphragmed fenestrae and PV-1 expression.

Conclusions:

The study confirms that adult GEnC largely lack diaphragmed fenestrae, unlike their embryonic counterparts.
The reappearance of PV-1 and diaphragmed fenestrae in adult GEnC during nephritis suggests a restorative remodeling process in the glomerular capillaries.
PV-1 expression and diaphragmed fenestrae may serve as valuable biomarkers for glomerular capillary remodeling after injury.