Related Experiment Video
Updated: Jul 5, 2026

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
Published on: May 25, 2017
Processing of Clostridium difficile toxins
Torsten Giesemann1, Martina Egerer1, Thomas Jank1
1Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Universität Freiburg, Albertstrasse 25, D-79104 Freiburg, Germany.
Abstract:
The pathogenicity of Clostridium difficile depends on the large clostridial glucosylating toxins A and B (TcdA and TcdB). The proteins accomplish their own uptake by a modular structure comprising a catalytic and a binding/translocation domain. Based on a proteolytic processing step solely the catalytic domain reaches the cytosol. Within the cells, the glucosyltransferases inactivate small GTPases by mono-O-glucosylation. Here, a short overview is given regarding latest insights into the intramolecular processing, which is mediated by an intrinsic protease activity.
Related Concept Videos
Bacterial Toxins
Botulism
Prevention of Further Absorption of Poison
Types of Toxins
Air pollutants, primarily gases, pose significant threats to respiratory health, leading to conditions like hypoxia, lung cancer, and in extreme cases, death.
Environmental pollutants like...
Bacterial Gastroenteritis
Diphtheria

