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Published on: May 6, 2015
[Construction of the recombinant adenovirus vector bearing dual-survivin shRNA]
Peng Gao1, Yu-Xi Shan, Dong-Rong Yang
1Department of Urology, Second Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215004, China. ag2525@tom.com
Objective:
To construct a recombinant adenovirus vector bearing dual-survivin short hairpin RNA (shRNA).
Methods:
Dual-survivin shRNA was designed and synthesized respectively, both inserted into adenovirus DNA. The recombinant adenovirus vector was confirmed via both sequencing and restriction digestion analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenoviruses.
Results:
The recombinant adenovirus vector was constructed and the target sequence was obtained.
Conclusion:
The construction of the recombinant adenovirus vector provides a basis for the research of potential gene therapy for prostate cancer.
