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Updated: Jul 5, 2026

Analysis of Fatty Acid Content and Composition in Microalgae
Published on: October 1, 2013
Determination of total sterols in brown algae by Fourier transform infrared spectroscopy
Naïma Bouzidi1, Yasmina Daghbouche, Mohamed El Hattab
1Laboratory of Medicinal and Aromatic Plants, University of Blida, P.O.B. 270 Soumâa (Blida 09000), Algeria.
Abstract:
A procedure is proposed for the determination of the total amount of sterols in brown algae Bifurcaria bifurcata, Cladostephus hirsitus, Dictyota dichotoma and Cystoseira sedoides, globally determined as fucosterol, which is the major sterol contained in these algae. The method involves the use of cholesterol as reference standard and a correction factor of 1.259+/-0.003, which represents the ratio between the slopes of calibration lines obtained from fucosterol and cholesterol. The method provides precise and accurate results for the IR analysis of real samples.
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