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Related Experiment Videos

La/SSB ribonucleoprotein levels increased in transformed cells.

R P Rother1, P S Thomas

  • 1Arthritis/Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.

Clinical and Experimental Immunology
|March 1, 1991
PubMed
Summary

Autoantibodies to La/SSB ribonucleoprotein are linked to Sjögren

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Area of Science:

  • Molecular Biology
  • Immunology
  • Cell Biology

Background:

  • Autoantibodies to La/SSB ribonucleoprotein are prevalent in Sjögren's syndrome patients.
  • La/SSB antigen is known to accumulate in cells following viral infection.

Purpose of the Study:

  • To investigate La/SSB antigen levels in virally and spontaneously transformed cell lines compared to their non-transformed counterparts.
  • To determine if elevated La/SSB is specific to viral transformation.

Main Methods:

  • Immunofluorescence assay to visualize La/SSB.
  • Flow cytometry to quantify La/SSB levels.
  • Capture ELISA to measure La/SSB antigen in cell lysates.

Main Results:

  • Transformed cell lines (BALB/3T12-3, KNRK) showed significantly higher La/SSB levels than their non-transformed counterparts (BALB/3T3, NRK) via immunofluorescence and flow cytometry.
  • Anti-Sm antibody and normal human serum did not show significant increases in the tested cell lines.
  • A two-fold increase in La/SSB antigen was confirmed in transformed cell lysates using capture ELISA.

Conclusions:

  • La/SSB antigen is elevated in transformed cell lines relative to non-transformed counterparts.
  • The observed increase in La/SSB is not limited to virally induced transformations.

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