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Human T cell responses to beta-galactosidase.

J D Oxley¹, R H Brookes, L S Rayfield

¹Department of Immunology, United Medical School, Guy's Hospital, London, England.

Clinical and Experimental Immunology

|March 1, 1991

Summary

Peripheral blood T cells naturally respond to beta-galactosidase (beta-Gal). Leucine methyl ester (LeuOMe) treatment enhanced B cell presentation of beta-Gal, suggesting its utility in human immunoregulation studies.

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Area of Science:

Immunology
Cell Biology

Background:

T cells in peripheral blood often exhibit natural responses to beta-galactosidase (beta-Gal).
This natural priming suggests beta-Gal as a relevant antigen for immunological studies.

Purpose of the Study:

To isolate and characterize T cell clones specific for beta-Gal.
To investigate the effect of leucine methyl ester (LeuOMe) treatment on antigen-presenting cells for beta-Gal.

Main Methods:

Isolation of T cell clones from peripheral blood mononuclear cells (PBMC), with and without LeuOMe pretreatment.
Characterization of T cell clones (CD4+, CD8-, alpha beta TcR+, cytotoxic potential).
Generation of Epstein-Barr virus (EBV) transformed B cell lines for antigen presentation assays.

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Main Results:

Four T cell clones specific for beta-Gal were successfully isolated.
LeuOMe-treated B cells demonstrated enhanced presentation of beta-Gal at lower antigen concentrations compared to untreated cells.
All isolated T cell clones expressed CD4, CD8-, and alpha beta TcR markers.

Conclusions:

Beta-galactosidase serves as a valuable model antigen for studying human immunoregulation.
LeuOMe pretreatment can enhance the efficiency of antigen presentation by B cells.
The findings support the role of natural T cell priming in immune responses.