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Updated: Jul 5, 2026

Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)
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Diffusive transport within dentinal tubules: an X-ray microtomographic study.

Masako Kawabata1, Mark P Hector, Graham R Davis

  • 1Centre for Oral Growth and Development, Barts and The London School of Medicine and Dentistry, Queen Mary, University of London, Mile End Road, London E1 4NS, UK.

Archives of Oral Biology
|May 20, 2008
PubMed
Summary

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Dentine hypersensitivity pain is linked to fluid movement in dentinal tubules. This study used X-ray microtomography to show caesium acetate primarily travels through these tubules, not side branches, confirming their role in pain signaling.

Area of Science:

  • Biomaterials Science
  • Dental Research
  • Biophysics

Background:

  • Dentine hypersensitivity is explained by the hydrodynamic theory, involving fluid movement in dentinal tubules.
  • Mechanosensitive nerves at the dentine-pulp interface are triggered by stimuli-induced fluid shifts.
  • Understanding fluid transport pathways is crucial for managing dentine hypersensitivity.

Purpose of the Study:

  • To investigate fluid transport pathways in dentine using X-ray microtomography (XMT).
  • To differentiate transport through primary dentinal tubules versus branched microtubules.
  • To validate the role of primary tubules in the hydrodynamic theory of dentine hypersensitivity.

Main Methods:

  • Coronal dentine disks (2.0 mm thick) were imaged using X-ray microtomography (XMT).

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Last Updated: Jul 5, 2026

Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)
08:09

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Published on: August 6, 2019

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  • Caesium acetate solution (0.50 mol L(-1)) was used to monitor diffusion into dentine over 1 and 6 days.
  • Changes in X-ray linear attenuation coefficient quantified caesium acetate ingress.

    • Main Results:

    • Significant caesium acetate ingress was observed beneath the exposed dentine surface.
    • Minimal ingress occurred beneath the sealed surface, indicating limited lateral transport.
    • Diffusive transport predominantly followed the direction of primary dentinal tubules.

    Conclusions:

    • Primary dentinal tubules are the main pathways for fluid transport in dentine.
    • Lateral transport through microtubules is not a significant factor in this process.
    • Primary tubules are key for triggering mechanosensitive nerves and delivering desensitizing agents.