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Updated: Jul 5, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Classical enterotoxigenic characteristics of Staphylococcus aureus strains isolated from bovine subclinical mastitis
Banur Boynukara1, Timur Gulhan, Mustafa Alisarli
1Department of Microbiology, Faculty of Veterinary Medicine, University of Yuzuncu Yil, 65080, Van, Turkey. banur61@hotmail.com
Abstract:
The aim of the present study was to investigate classical enterotoxigenic properties of Staphylococcus aureus strains isolated from cows with subclinical mastitis. For this purpose, 480 milk samples from cows with subclinical mastitis raised in different villages neighbouring Van city Center were collected. A total of 106 S. aureus strains were isolated. Twenty seven isolates (25.5%) were found to be enterotoxigenic by reverse passive latex agglutination (RPLA). Of these, 25 (23.6%) were positive for staphylococcal enterotoxin A (SEA), 2 (1.9%) for SEB. None of the isolates was positive for SEC or SED. This study showed that most S. aureus strains isolated from bovine subclinical mastitis produced SEA compared to other SEs.
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