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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
DNA detection using nanostructured SERS substrates with Rhodamine B as Raman label
Cheng Fang1, Ajay Agarwal, Kavitha Devi Buddharaju
1Institute of Microelectronics, A STAR (Agency for Science, Technology and Research), Singapore Science Park II, Singapore. fangc@ime.a-star.edu.sg
Biosensors & Bioelectronics
|May 20, 2008
Summary
This study presents a novel Surface-Enhanced Raman Scattering (SERS) method for detecting DNA hybridization. The technique achieves a low detection limit of 1 x 10(-12)M using specialized silicon nanostructures.
Area of Science:
- Nanotechnology
- Biochemistry
- Spectroscopy
Background:
- Detecting DNA hybridization at low concentrations is crucial for diagnostics.
- Existing methods may lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop a sensitive and reliable method for DNA hybridization detection.
- To utilize Surface-Enhanced Raman Scattering (SERS) with novel nanostructures for enhanced sensitivity.
Main Methods:
- Fabrication of silicon nanostructures coated with gold-silver for SERS substrates.
- Hybridization of target DNA with Peptide Nucleic Acids (PNA) in nanogaps.
- Utilizing a zirconium (Zr(4+)) linker and Rhodamine B (RB) Raman label for signal amplification.
Main Results:
- Demonstrated successful DNA hybridization detection using the SERS substrate.
- Achieved a highly sensitive detection limit of 1 x 10(-12)M for target DNA.
- The method showed specificity, as the linker was only incorporated upon DNA hybridization.
Conclusions:
- The developed SERS technique offers a sensitive platform for DNA hybridization detection.
- Silicon nanostructures with gold-silver coating provide an effective substrate for SERS-based biosensing.
- This method holds potential for various applications requiring low-concentration DNA detection.
