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Updated: Jul 5, 2026
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
Published on: June 11, 2019
Optimization of an effective directed differentiation medium for differentiating mouse bone marrow mesenchymal stem
Xiao-Lei Shi1, Liang Mao, Bi-Yun Xu
1Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical College of Nanjing University, No. 321 Zhongshan Road, Nanjing 210008, PR China.
Abstract:
We have used a uniform design to explore the most effective directed differentiation medium (MEDDM) for differentiating mouse bone marrow mesenchymal stem cells (mMSCs) into hepatocytes. Our methods involved arranging eight differentiation medium groups following uniform design. Flow cytometry was used to evaluate the percentage of ALB+ and CK18+ cells in each group. Factors and their concentrations in the MEDDMs were then identified. The MEDDMs were evaluated by their ability to differentiate mMSCs into hepatocytes by RNA and protein expressions and synthesis functions. FGF at 35 ng/ml and OSM at 30 ng/ml in the medium yielded the highest percentage of ALB+ and CK18+ cells. During directed differentiation using MEDDMs, ALB, CK18, TTR, AFP mRNAs were expressed. ALB and CK18 proteins were detected in the cells. The differentiated cells produced albumin and urea in a time dependent manner. Uniform design was adequate for choosing the MEDDM of mMSCs. MEDDM containing 35 ng/ml FGF and 30 ng/ml OSM was effective in differentiating mMSCs into hepatocytes.
