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FVB/N: an inbred mouse strain preferable for transgenic analyses.

M Taketo¹, A C Schroeder, L E Mobraaten

¹Jackson Laboratory, Bar Harbor, ME 04609.

Proceedings of the National Academy of Sciences of the United States of America

|March 15, 1991

Summary

The FVB/N mouse strain is ideal for transgenic research due to its reproductive efficiency and large pronuclei in zygotes, facilitating DNA microinjection. This strain demonstrates superior survival and transgenic efficiency compared to other common mouse models.

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Area of Science:

* Genetics and Genomics
* Animal Models in Research
* Transgenic Technology

Background:

* The FVB/N mouse strain is a widely used inbred strain for genetic research.
* Key characteristics include high reproductive performance and large litter sizes.
* FVB/N zygotes possess large pronuclei, advantageous for DNA microinjection.

Purpose of the Study:

* To evaluate the suitability of FVB/N mice for transgenic experiments.
* To compare the efficiency of generating transgenic mice using FVB/N zygotes versus other strains.
* To analyze genetic markers of the FVB/N strain.

Main Methods:

* Microinjection of DNA constructs into zygotes of FVB/N, C57BL/6J, and (C57BL/6J x SJL/J)F1 strains.
* Assessment of zygote survival rates post-injection.

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* Analysis of genetic markers across 15 chromosomes in FVB/N mice.

Main Results:

* FVB/N zygotes exhibited high survival rates after DNA microinjection.
* Transgenic mice were generated with efficiencies comparable to F1 hybrids and significantly higher than C57BL/6J.
* Genetic marker analysis confirmed the FVB/N strain's utility for genetic studies.

Conclusions:

* The FVB/N strain is highly advantageous for transgenic mouse production due to its reproductive traits and zygote characteristics.
* Its genetic makeup and efficiency in generating transgenic models make it a preferred choice for research.
* Ongoing development of congenic strains further enhances its research applications.