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Updated: Jul 5, 2026

Quantification of Acanthamoeba spp. Motility
Published on: September 20, 2024
Acanthamoeba castellanii: identification and distribution of actin cytoskeleton
Arturo González-Robles1, Guadalupe Castañón, Verónica Ivonne Hernández-Ramírez
1Department of Experimental Pathology, Center for Research and Advanced Studies, Mexico City, Mexico. goroa@cinvestav.mx
Abstract:
The presence of the cytoskeleton of Acanthamoeba castellanii was observed by means of cryo-electronmicroscopy and immunofluorescence techniques. This structure is formed largely by fibers and networks of actin located mainly in cytoplasmic locomotion structures as lamellipodia and as well as in various endocytic structures. In addition, the comparison between total actin content in whole extracts among different amoebae was made. The molecular weight of actin in A. castellanii was 44 kDa, and 45 kDa for Naegleria fowleri and Entamoeba histolytica.
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