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Updated: Jul 5, 2026

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

Fine expression profiling of full-length transcripts using a size-unbiased cDNA library prepared with the

Mio Oshikawa¹, Yoshiko Sugai, Ron Usami

¹Department of Rehabilitation Engineering, Research Institute, National Rehabilitation Center for Persons with Disabilities, 4-1 Namiki, Tokorozawa, Saitama 359-8555, Japan.

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes

|May 20, 2008

Summary

A novel vector-capping method creates high-quality, full-length complementary DNA (cDNA) libraries. This size-unbiased library is ideal for detailed analysis of gene expression, including alternative splicing and transcriptional start sites.

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Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

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Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

Area of Science:

Molecular Biology
Genomics
Transcriptomics

Background:

Full-length complementary DNA (cDNA) libraries are crucial for understanding gene expression.
Traditional methods often face challenges with library size bias and completeness.

Purpose of the Study:

To analyze the characteristics of a full-length cDNA library constructed using the vector-capping method.
To evaluate the efficiency and utility of this method for transcriptomic studies.

Main Methods:

Construction of a size-unbiased, full-length cDNA library using the vector-capping method.
Single-pass sequencing of 24,000 randomly selected clones from an unamplified library.
Bioinformatic analysis including genome mapping and identification of transcriptional units.

Main Results:

Over 95% of the sequenced clones represented full-length cDNAs.
Identified 4,513 transcriptional units, including 36 antisense transcripts.
Detected numerous long-sized cDNAs and multiple splicing variants, demonstrating the library's capacity for capturing complex transcriptomes.

Conclusions:

The vector-capping method yields a highly efficient, size-unbiased full-length cDNA library.
This resource is suitable for in-depth expression profiling, alternative splicing, and alternative transcriptional start site analysis.