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Related Experiment Video

Updated: Jul 5, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

QUMA: quantification tool for methylation analysis.

Yuichi Kumaki1, Masaaki Oda, Masaki Okano

  • 1Laboratory for Mammalian Epigenetic Studies, Center for Developmental Biology, RIKEN, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.

Nucleic Acids Research
|May 20, 2008
PubMed
Summary

Researchers developed QUMA, a web tool simplifying DNA methylation analysis from bisulfite sequencing data. This tool streamlines complex data processing and enhances quality control for more reliable results.

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Area of Science:

  • Epigenetics
  • Bioinformatics

Background:

  • Bisulfite sequencing is a key method for DNA methylation profiling in research and clinics.
  • Current analysis is hampered by time-consuming processes and subjective quality control.

Purpose of the Study:

  • To develop an efficient and user-friendly web-based tool for bisulfite sequencing data analysis.
  • To provide consistent quality control for DNA methylation profiling.

Main Methods:

  • Development of QUMA (Quantification Tool for Methylation Analysis), an interactive web server.
  • Integration of essential data-processing functions for bisulfite sequence analysis.
  • Implementation of a platform for standardized quality control.

Main Results:

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DNA Methylation: Bisulphite Modification and Analysis
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DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Related Experiment Videos

Last Updated: Jul 5, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

  • QUMA offers a streamlined workflow for analyzing CpG methylation from bisulfite sequencing.
  • The tool facilitates consistent and less biased quality checking of results.
  • Provides a centralized platform for comprehensive methylation analysis.

    • Conclusions:

    • QUMA addresses the limitations of traditional bisulfite sequencing analysis.
    • The tool enhances accuracy and reproducibility in DNA methylation studies.
    • QUMA is accessible online at http://quma.cdb.riken.jp/ for broader research use.