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PCR specific for Actinobacillus pleuropneumoniae serotype 3

L Zhou1, S C P Jones, Ø Angen

  • 1Department of Paediatrics, Imperial College London, St Mary's Campus, London w2 1pg.

The Veterinary Record
|May 20, 2008
PubMed

Insights

A new PCR test accurately identifies Actinobacillus pleuropneumoniae serotype 3, improving diagnosis of swine respiratory disease. This method overcomes limitations of traditional serotyping, aiding UK epidemiological surveys.

Area of Science:

  • Veterinary Microbiology
  • Molecular Diagnostics
  • Bacteriology

Background:

  • Actinobacillus pleuropneumoniae causes significant porcine pleuropneumonia, with serotypes 3 and 8 prevalent in the UK.
  • Traditional serotyping methods for A. pleuropneumoniae exhibit cross-reactivity issues between serotypes 3, 6, and 8.

Purpose of the Study:

  • To develop a highly specific and sensitive PCR assay for identifying Actinobacillus pleuropneumoniae serotype 3.
  • To enable multiplex PCR application with the apxIV gene for improved diagnostic capabilities.

Main Methods:

  • Development of a serotype 3-specific PCR targeting the capsule locus.
  • Evaluation of the PCR assay on 266 A. pleuropneumoniae strains and 121 strains of other relevant bacterial pathogens.
  • Validation of a multiplex PCR format incorporating the apxIV gene.

Main Results:

  • The developed PCR demonstrated high specificity and sensitivity in differentiating A. pleuropneumoniae strains.
  • The assay successfully distinguished between serotypes 3, 6, and 8, overcoming cross-reactivity issues.
  • The multiplex PCR format proved effective for simultaneous detection.

Conclusions:

  • The novel PCR assay is a valuable tool for accurate identification of A. pleuropneumoniae serotype 3.
  • This method enhances differentiation of closely related serotypes, crucial for epidemiological studies in regions like the UK.
  • The assay supports improved seroprevalence and epidemiological surveys for swine respiratory disease.

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