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PCR specific for Actinobacillus pleuropneumoniae serotype 3.
1Department of Paediatrics, Imperial College London, St Mary's Campus, London w2 1pg.
The Veterinary Record
|May 20, 2008
Summary
A new PCR test accurately identifies Actinobacillus pleuropneumoniae serotype 3, improving diagnosis of swine respiratory disease. This method overcomes limitations of traditional serotyping, aiding UK epidemiological surveys.
Area of Science:
- Veterinary Microbiology
- Molecular Diagnostics
- Bacteriology
Background:
- Actinobacillus pleuropneumoniae causes significant porcine pleuropneumonia, with serotypes 3 and 8 prevalent in the UK.
- Traditional serotyping methods for A. pleuropneumoniae exhibit cross-reactivity issues between serotypes 3, 6, and 8.
Purpose of the Study:
- To develop a highly specific and sensitive PCR assay for identifying Actinobacillus pleuropneumoniae serotype 3.
- To enable multiplex PCR application with the apxIV gene for improved diagnostic capabilities.
Main Methods:
- Development of a serotype 3-specific PCR targeting the capsule locus.
- Evaluation of the PCR assay on 266 A. pleuropneumoniae strains and 121 strains of other relevant bacterial pathogens.
- Validation of a multiplex PCR format incorporating the apxIV gene.
Main Results:
- The developed PCR demonstrated high specificity and sensitivity in differentiating A. pleuropneumoniae strains.
- The assay successfully distinguished between serotypes 3, 6, and 8, overcoming cross-reactivity issues.
- The multiplex PCR format proved effective for simultaneous detection.
Conclusions:
- The novel PCR assay is a valuable tool for accurate identification of A. pleuropneumoniae serotype 3.
- This method enhances differentiation of closely related serotypes, crucial for epidemiological studies in regions like the UK.
- The assay supports improved seroprevalence and epidemiological surveys for swine respiratory disease.
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