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Purification of Hsp104, a Protein Disaggregase
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Protein disaggregation by the AAA+ chaperone ClpB involves partial threading of looped polypeptide segments

Tobias Haslberger1, Agnieszka Zdanowicz, Ingo Brand

  • 1Zentrum für Molekulare Biologie Heidelberg, DKFZ-ZMBH Alliance, Universität Heidelberg, Im Neuenheimer Feld 282, Heidelberg D-69120, Germany.

Nature Structural & Molecular Biology
|May 20, 2008
PubMed
Summary

The ClpB chaperone system, with DnaK, efficiently reactivates aggregated proteins by threading misfolded parts. This process works even for complex protein fusions, showing ClpB

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