Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

Fertilization

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Patient-derived organotypic tumor spheroids as a functional platform for predicting immunotherapeutic responses and guiding precision treatment for hepatocellular carcinoma.

Journal for immunotherapy of cancer·2026

Same author

Higher glycated hemoglobin amplifies the effect of apolipoprotein E epsilon 4-related cognition and olfaction impairments in type 2 diabetes.

World journal of diabetes·2025

Same author

Subcutaneous delivery of icariin via a gelatin methacryloyl (GelMA) hydrogel sustained-release system improves ovarian function in reproductively aged mice.

Zoological research·2025

Same author

Basal Forebrain to Ventral Tegmental Area Glutamatergic Pathway Promotes Emergence from Isoflurane Anesthesia in Mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025

Same author

Role of the Supramammillary Nucleus-Medial Septum Glutamatergic Pathway in Mediating the Effects of Isoflurane Anesthesia.

Anesthesiology·2025

Same author

<sup>18</sup>F-FDG PET/CT Predicts the Prognosis of Patients with Hepatocellular Carcinoma Undergoing Liver Transplantation.

Liver cancer·2025

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

[Sperm DNA damage and assisted reproductive technology].

Zhi-Peng Xu¹, Hai-Xiang Sun, Ning-Yuan Zhang

¹Center of Reproductive Medicine, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China. xzpbio@hotmail.com

Zhonghua Nan Ke Xue = National Journal of Andrology

|May 21, 2008

Summary

Sperm DNA damage is a key indicator of male fertility, impacting assisted reproductive technology (ART) success. Strategies exist to reduce DNA damage, improving ART outcomes and reducing risks for ICSI offspring.

More Related Videos

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

Published on: May 17, 2024

Related Experiment Videos

Last Updated: Jul 5, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

Published on: May 17, 2024

Area of Science:

Reproductive biology and genetics.
Andrology and male infertility research.
Assisted reproductive technologies (ART).

Context:

Sperm assessment has evolved beyond routine semen analysis with novel cellular and molecular measures.
Sperm DNA damage is recognized as a significant marker for male fertility.
Techniques like Sperm Chromatin Structure Assay (SCSA) are used to quantify sperm DNA damage.

Purpose:

To review the mechanisms and detection methods of sperm DNA damage.
To explore the association between sperm DNA damage and reproductive outcomes, including ART success and spontaneous abortion.
To discuss treatment strategies aimed at reducing sperm DNA damage and improving ART efficacy.

Summary:

Sperm DNA damage arises from issues like abnormal chromatin packaging, oxidative stress, and apoptosis.
Elevated sperm DNA damage is linked to adverse ART outcomes, recurrent miscarriages, and potential genetic risks for ICSI-conceived offspring.
Potential treatments include antioxidants, testicular sperm ICSI, sperm cryopreservation, etiological factor removal, and traditional Chinese medicine.

Impact:

Provides a comprehensive overview of sperm DNA damage in the context of ART.
Highlights the clinical relevance of sperm DNA damage assessment for male fertility evaluation.
Informs potential therapeutic interventions to enhance fertility and ART success rates.