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Comparison of vesicular stomatitis virus defective interfering particle synthesis in chick embryo and L cells
Abstract:
A comparison of the ability of vesicular stomatitis virus (VSV) to generate and replicate defective interfering (DI) particles in primary chick embryo (CE) and mouse L cells was investigated as a means of analyzing host control over DI-particle synthesis and interfering capacity. Serial undiluted passage of VSV in CE and L cells indicate that VSV-DI particles are generated and (or) replicate with greater efficiency in CE than in L cells. When DI particles accumulate in L cells, they are able to interfere with infectious particle replication. The DI particles from CE cells interfered to the same extent with infectious particle replication in both CE and L cells. L cells, therefore, are not considered 'low-interference' hosts in which DI particles are produced and do not interfere with infectious virus replication, but rather hosts which restrict the production of DI particles.
Insights
Vesicular stomatitis virus (VSV) generates defective interfering (DI) particles more efficiently in chick embryo cells than in mouse L cells. Mouse L cells restrict DI particle production, rather than their interference capacity.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Defective interfering (DI) particles are subviral particles that arise during viral replication.
- DI particles can interfere with the replication of standard virus.
- Host cell control over DI particle generation and interference is not fully understood.
Purpose of the Study:
- To compare the generation and replication efficiency of VSV-DI particles in chick embryo (CE) and mouse L cells.
- To analyze host cell control over DI particle synthesis and interfering capacity.
Main Methods:
- Serial undiluted passage of Vesicular Stomatitis Virus (VSV) in primary chick embryo (CE) and mouse L cells.
- Assessment of DI particle generation, replication, and interference capacity.
Main Results:
- VSV-DI particles were generated and replicated with greater efficiency in CE cells compared to L cells.
- DI particles accumulating in L cells interfered with infectious particle replication.
- DI particles originating from CE cells exhibited similar interference levels in both CE and L cells.
Conclusions:
- Mouse L cells restrict the production of VSV-DI particles.
- L cells are not 'low-interference' hosts but rather hosts that limit DI particle synthesis.