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Updated: Jul 5, 2026
Synthesis of an In vivo MRI-detectable Apoptosis Probe
Published on: July 31, 2012
Synthesis, biological evaluation and radiochemical labeling of a dansylhydrazone derivative as a potential imaging
Wenbin Zeng1, Min-Liang Yao, David Townsend
1Molecular Imaging and Translational Research Program, University of Tennessee, Graduate School of Medicine, Box 93, 1924 Alcoa Highway, Knoxville, TN 37920, USA.
Abstract:
To develop a small molecule-based tracer for in vivo apoptosis imaging, dansylhydrazone (DFNSH) was synthesized in 93% yield in less than 30 min. The biological evaluation showed that DFNSH selectively binds to paclitaxel-induced apoptotic cancer cells. The high magnification fluorescent images demonstrate that DFNSH is localized within the cytoplasm of cells that bound Alexa 488 labeled annexin V on the plasma membrane. [(18)F]-DFNSH ([(18)F]-3) was synthesized and isolated in 50-60% radiochemical yields, based on [K/K(222)](18)F, with a synthesis time of 50 min (EOB). The straightforward preparation of fluorine-18 labeled 3 makes it a promising tracer for PET imaging of apoptosis.
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