Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammatory Bowel Disease III: Crohn's Disease01:25

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two Blood-based Endotypes Reveal Divergent Clinical Outcomes of Fibrotic Hypersensitivity Pneumonitis.

medRxiv : the preprint server for health sciences·2026
Same author

Quantitative molecular cartography of emergency myelopoiesis reveals conserved modules of hematopoietic activation.

Cell stem cell·2026
Same author

Regulatory B cells contribute to allergen-encapsulating nanoparticle immunotherapy efficacy for food allergy.

JCI insight·2026
Same author

Myeloid Cell-Targeting PLGA Nanoparticles Ameliorate Acute Graft-Versus-Host Disease.

Cancers·2026
Same author

Reply to Fiorentù et al.: Lymphoid follicles in the lung complement lymph node activation in IPF.

American journal of respiratory and critical care medicine·2026
Same author

Negative regulation of human IL-33 in endothelium during allergic airway inflammation.

JCI insight·2026
Same journal

The scaffolding protein AKAP79/150 shapes innate immune responses to allergen.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Optineurin restrains IL-17-associated neuroinflammation in trigeminal ganglia to preserve sensory function after ocular HSV-1 infection.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Crystal structure and immune single-cell atlas provide insights into the functional divergence of type I IFNs in fish.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Related Experiment Video

Updated: Jul 5, 2026

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

CD43 regulates Th2 differentiation and inflammation.

Judy L Cannon1, Amélie Collins, Purvi D Mody

  • 1Department of Medicine, Section of Pulmonary and Critical Care Medicine, University of Chicago, Chicago, IL 60637, USA. jlcanno@uchicago.edu

Journal of Immunology (Baltimore, Md. : 1950)
|May 21, 2008
PubMed
Summary
This summary is machine-generated.

Mice lacking CD43 (CD43-/-) show enhanced T helper 2 (Th2) cell differentiation and increased allergic airway inflammation. However, this CD43 deficiency does not alter Th1-mediated autoimmune disease progression.

More Related Videos

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Related Experiment Videos

Last Updated: Jul 5, 2026

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD43 is a transmembrane protein crucial for T cell activation.
  • Studies suggest CD43's cytoplasmic tail influences T cell proliferation intracellularly.
  • CD43 deficiency leads to hyperproliferation in T cells.

Purpose of the Study:

  • To investigate the role of CD43 in T cell activation and differentiation.
  • To determine the impact of CD43 deficiency on Th1 and Th2 mediated immune responses in vivo and in vitro.
  • To elucidate the intracellular mechanisms affected by CD43 absence.

Main Methods:

  • T cell activation assays upon T cell receptor (TCR) ligation.
  • Analysis of tyrosine phosphorylation and calcium flux in CD43(-/-) T cells.
  • In vitro differentiation assays for Th2 cell polarization.
  • In vivo studies using mouse models for Th2-mediated allergic airway disease and Th1-mediated autoimmune diabetes.
  • Experimental autoimmune encephalomyelitis (EAE) induction.

Main Results:

  • CD43(-/-) T cells showed no increase in tyrosine phosphorylation but a decreased calcium flux upon TCR ligation.
  • CD43(-/-) T cells preferentially differentiated into Th2 cells, with increased GATA-3 nuclear translocation.
  • CD43(-/-) mice exhibited exacerbated inflammation in Th2-mediated allergic airway disease models.
  • In contrast, CD43(-/-) mice showed normal disease onset and progression in Th1-mediated diabetes and EAE models.
  • Despite normal Th1 responses, CD43(-/-) mice produced more IL-5 in vitro and had decreased delayed-type hypersensitivity responses.

Conclusions:

  • CD43 deficiency promotes Th2 cell differentiation and exacerbates Th2-driven inflammation.
  • The absence of CD43 does not impair Th1-mediated autoimmune responses.
  • Preferential Th2 differentiation in CD43(-/-) T cells is insufficient to protect against Th1-mediated autoimmunity.