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Isolation and biochemical characterization of a zona pellucida-binding glycoprotein of boar spermatozoa
V Jonákova1, L Sanz, J J Calvete
1Institute of Molecular Genetics, Prague, Czechoslovakia.
FEBS Letters
|March 11, 1991
Abstract:
Lectin-like molecules on the sperm surface are implicated in the process of gamete recognition and adhesion. We have isolated and biochemically characterized a 15 kDa glycoprotein from ejaculated boar sperm which possess zona pellucida-binding- and haemagglutinating-activity. The zona/15 kDa protein interaction is inhibited by fucoidan, suggesting that the glycoprotein is one of the sperm components which participate in the initial gamete interaction. N-Terminal sequence analysis of the isolated 15 kDa glycoprotein showed that it may belong to the same sperm/egg recognition-mediating protein family as the sea urchin sperm protein binding.