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Radiolabelled granulocytes in inflammatory bone disease
P D'Amico1, S Lastoria, N Caccavella
1Department of Nuclear Medicine, University of Naples, Italy.
International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology
|January 1, 1991
Summary
This study shows that using a labelled monoclonal antibody to target white blood cells is effective for visualizing inflammatory lesions. The method provides excellent scintigram quality and is suitable for acute diagnosis.
Area of Science:
- Nuclear Medicine
- Immunoscintigraphy
- Oncoimmunology
Background:
- Inflammatory lesions require accurate diagnostic methods.
- In vivo imaging of inflammatory processes is crucial for patient management.
- Monoclonal antibodies offer targeted delivery for diagnostic agents.
Purpose of the Study:
- To evaluate the efficacy of a 99mTc-labelled monoclonal antibody for in vivo imaging of inflammatory lesions.
- To assess the diagnostic quality and timing of scintigraphy using labelled granulocytes.
- To determine the suitability of this method for acute diagnostic settings.
Main Methods:
- Investigated 7 patients with inflammatory lesions.
- Utilized a monoclonal antibody (mouse IgG1, BW 250/183) targeting NCA and CEA.
- Administered the 99mTc-labelled antibody intravenously.
- Acquired scintigrams at 3-6 hours and 24 hours post-injection.
Main Results:
- All inflammatory lesions were successfully visualized.
- Scintigrams demonstrated excellent image quality.
- Optimal visualization was achieved between 3-6 and 24 hours after injection.
- The antibody can be stored freeze-dried and labelled with 99mTc without cell separation.
Conclusions:
- The 99mTc-labelled monoclonal antibody effectively visualizes inflammatory lesions.
- The method provides high-quality scintigraphy suitable for clinical use.
- The ease of preparation and storage makes this technique valuable for acute diagnosis.