Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

The K/BxN arthritis model.

Paul A Monach¹, Diane Mathis¹, Christophe Benoist¹

¹Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts.

Current Protocols in Immunology

|May 21, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Type I interferon primes the alveolar epithelium to receive reparative signals from tissue-resident macrophages.

bioRxiv : the preprint server for biology·2026

Same author

Author Correction: Piezo1-dependent activation of stromal cells ignites muscle inflammation in exercise and injury and is associated with inflammaging.

Nature immunology·2026

Same author

Piezo1-dependent activation of stromal cells ignites muscle inflammation in exercise and injury and is associated with inflammaging.

Nature immunology·2026

Same author

Regulatory T cells safeguard liver health during metabolic-associated steatohepatitis.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Immunological knowledge.

Nature immunology·2026

Same author

The history and promise of Treg cells.

Immunity·2025

Same journal

A Comprehensive Experimental Guide to Studying Cross-Presentation in Dendritic Cells In Vitro.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Monoclonal Antibodies to Human Cell Surface Antigens.

Current protocols in immunology·2020

Same journal

Protocols for Experimental Sjögren's Syndrome.

Current protocols in immunology·2020

See all related articles

K/BxN mice develop severe inflammatory arthritis due to autoantibodies against glucose-6-phosphate isomerase (GPI). Transferring serum from these mice induces arthritis in other strains, aiding research into autoimmunity and joint inflammation mechanisms.

Area of Science:

Immunology
Autoimmunity
Inflammation

Background:

K/BxN mice, expressing specific T cell receptor (TCR) and MHC class II molecules, spontaneously develop severe inflammatory arthritis.
Serum from K/BxN mice can transfer this arthritis to other mouse strains, mediated by autoantibodies targeting glucose-6-phosphate isomerase (GPI).
This serum transfer model is crucial for studying the mechanisms of joint-specific inflammation induced by anti-GPI autoantibodies.

Purpose of the Study:

To provide detailed methods for maintaining K/BxN mouse colonies.
To outline procedures for inducing arthritis via serum transfer.
To describe methods for the clinical evaluation of arthritis and measurement of anti-GPI antibodies.

Main Methods:

Maintenance of a K/BxN transgenic mouse colony.

Related Experiment Videos

Last Updated: Jul 5, 2026

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

Induction of inflammatory arthritis through serum transfer.

Clinical assessment of joint inflammation and severity.

Quantification of anti-GPI autoantibody levels.

Main Results:

Established protocols for K/BxN mouse colony management.
Demonstrated successful induction of arthritis via serum transfer.
Detailed methods for arthritis evaluation and antibody measurement.

Conclusions:

The K/BxN serum transfer model is a valuable tool for investigating autoimmune arthritis.
This unit provides essential methodologies for researchers studying anti-GPI autoantibody-mediated joint inflammation.
Standardized methods facilitate reproducible research in autoimmunity and inflammatory arthritis.