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Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
Proteomic map of peripheral blood mononuclear cells
Daniele Vergara1, Fernanda Chiriacò, Raffaele Acierno
1Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
Proteomics
|May 21, 2008
Summary
Researchers created a database of human peripheral blood mononuclear cells (PBMCs) proteins. This proteomics resource aids in understanding cellular changes linked to diseases like cancer and autoimmune disorders.
Area of Science:
- Proteomics
- Cellular Biology
- Biochemistry
Background:
- Extensive research in proteomics focuses on identifying proteins in various cell types.
- Significant advancements have been made in characterizing blood cellular components.
Purpose of the Study:
- To establish a public two-dimensional gel electrophoresis (2-DE) database for human peripheral blood mononuclear cells (PBMCs) proteins.
- To provide a resource for investigating proteomics changes associated with diseases.
Main Methods:
- Isolation of PBMCs from six healthy individuals.
- Separation and identification of proteins using 2-DE.
- Classification and annotation of identified proteins.
Main Results:
- Identification of 174 distinct proteins from 246 protein spots on 2-DE gels.
- Categorization of identified proteins into thirteen functional or localization-based groups.
- Annotation of protein data available at http://physiology.unile.it/proteomics.
Conclusions:
- The established PBMC proteomics database serves as a valuable resource.
- PBMCs have potential for studying proteomics alterations in various pathologies.
- This resource can aid research in autoimmune, neurodegenerative, and cancer diseases.
