Virus-infected avian cell lines established in vitro

Area of Science:

• * Avian virology
• * Cell culture
• * Oncology

Background:

• * Avian leukosis viruses (ALVs) are significant pathogens in poultry, causing various tumors and economic losses.
• * Establishing continuous cell lines from virus-infected avian sources is crucial for studying viral pathogenesis and oncogenesis.

Purpose of the Study:

• * To establish and characterize avian cell lines infected with BAI strain A virus and MC29 avian leukosis virus.
• * To evaluate the oncogenic potential and viral release characteristics of these cell lines in vivo.
• * To assess the suitability of these cell lines for long-term culture and research.

Main Methods:

• * Establishment of four virus-infected avian cell lines in continuous culture.
• * Inoculation of cell lines into young chicks to assess disease induction (myeloblastic leukemia or tumors).
• * Chromosome analysis to determine karyotypes and identify genetic alterations.

Main Results:

• * Two cell lines infected with BAI strain A virus produced low levels of infectious virus but induced myeloblastic leukemia less efficiently than primary myeloblasts.
• * Two cell lines infected with MC29 avian leukosis virus released normal virus levels and caused rapid tumor development at inoculation sites in chicks.
• * All four cell lines possessed typical Gallus domesticus karyotypes, with myeloblastic lines showing microchromosome reduction; they demonstrated stability, cryopreservation viability, and potential for indefinite growth.

Conclusions:

• * The developed cell lines serve as valuable in vitro models for studying avian leukosis viruses and their oncogenic mechanisms.
• * The MC29-infected cell lines are particularly useful for rapid tumor induction studies.
• * These cell lines are robust, amenable to long-term storage and continuous propagation, facilitating future research.