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Isolation of Preadipocytes from Broiler Chick Embryos
Published on: August 4, 2022
Increased glucose availability activates chicken thymocyte metabolism and survival
Brooke D Humphrey1, Shashidhara G Rudrappa
1Department of Animal and Avian Sciences, University of Maryland College Park, MD 20742, USA. bdhumphr@calpoly.edu
The Journal of Nutrition
|May 22, 2008
Summary
Chicken thymocyte glucose metabolism and development are linked to glucose availability. Higher glucose concentrations enhance thymocyte energy status and survival, influencing naïve T cell development.
Area of Science:
- Immunology
- Cell Biology
- Metabolic Research
Background:
- Mammalian thymocyte development is linked to glucose metabolism.
- Chicken thymocytes face varying glucose concentrations during development (low in ovo, high posthatch).
Purpose of the Study:
- To investigate the impact of glucose concentration on chicken thymocyte metabolism, energy status, and survival.
- To understand how glucose availability influences thymocyte development in chickens.
Main Methods:
- Culturing embryonic chicken thymic lobes in media with varying glucose concentrations.
- Isolating thymocytes for analysis of glucose transporter mRNA (Glut-1, Glut-3), glucose uptake, hexokinase activity, mitochondrial membrane potential, viability, and apoptosis.
Main Results:
- Increased glucose transporter mRNA (Glut-1, Glut-3) and glucose uptake at higher glucose concentrations (10-20 mmol/L).
- Hexokinase activity was highest at 0 and 20 mmol/L glucose.
- Higher glucose concentrations (15 mmol/L) increased mitochondrial membrane potential, indicating enhanced energy status.
- Lower glucose concentrations (5 mmol/L) reduced thymocyte viability and increased apoptosis.
Conclusions:
- Chicken thymocyte glucose utilization is dependent on glucose availability.
- Glucose availability significantly alters thymocyte energy status and survival.
- Glucose concentration plays a crucial role in regulating chicken thymocyte development and potentially naïve T cell differentiation.
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