Related Experiment Video
Updated: Jul 5, 2026
08:48
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
Published on: January 26, 2017
Saccharomyces cerevisiae Yta7 regulates histone gene expression
Angeline Gradolatto1, Richard S Rogers, Heather Lavender
1Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Genetics
|May 22, 2008
Summary
The Saccharomyces cerevisiae Yta7 protein helps silence chromatin by regulating histone gene transcription. It binds histone H3
Area of Science:
- Molecular Biology
- Chromatin Biology
- Yeast Genetics
Background:
- The Saccharomyces cerevisiae Yta7 protein is part of a chromatin complex maintaining transcriptional zones.
- Yta7 was previously found to associate with Spt16, a member of the yFACT complex.
Purpose of the Study:
- To investigate the functional relationship between Yta7, Spt16, and the histone regulatory pathway.
- To elucidate the mechanism by which Yta7 influences chromatin structure and gene transcription.
Main Methods:
- Epistasis analyses to determine genetic interactions between Yta7, Spt16, and histone regulatory factors.
- Cell-cycle analysis to assess the timing of Yta7's regulatory role.
- Chromatin immunoprecipitation and biochemical assays to investigate Yta7's association with histone genes and histone H3.
Main Results:
- Epistasis studies revealed a link between Yta7, Spt16, and known histone regulatory pathway components.
- Yta7 regulates histone gene transcription in a cell-cycle-dependent manner.
- Yta7's bromodomain-like region binds the N-terminal tail of histone H3 at histone gene loci.
Conclusions:
- Yta7 localizes to chromatin to establish transcriptional silencing.
- A key function of Yta7 involves modulating the transcription of histone genes.
- The interaction with histone H3 is crucial for Yta7's role in chromatin regulation.
Related Concept Videos
01:28Yeast Signaling
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
01:17Gene Regulation During Sporulation
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
02:30Histone Variants at the Centromere
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...