Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

A simple strategy for the creation of a recombinant lectin microarray.

Ku-Lung Hsu1, Jeffrey C Gildersleeve, Lara K Mahal

  • 1Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station, A5300, Austin, TX 78712-0265, USA.

Molecular Biosystems
|May 22, 2008
PubMed
Summary

Researchers developed recombinant lectins for advanced glycomic analysis using lectin microarrays. This overcomes limitations of natural lectins, enabling reliable carbohydrate-binding protein detection and glycopattern analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same author

High-Speed AFM Reveals Ligand-Dependent Supramolecular Switching of Human Phosphofructokinase-1.

Research square·2026
Same author

The Q226H Mutation in Avian H5N1 Hemagglutinin Mediates a Path towards Structural Adaptation in Humans.

bioRxiv : the preprint server for biology·2026
Same author

A chemoproteomic atlas of the human purine interactome for regioselective ligand discovery.

Nature communications·2026
Same author

Unraveling the Molecular Basis of Cooperativity in Cholera Toxin-Glycan Interactions.

Analytical chemistry·2026
Same author

microRNAs bidirectionally regulate FUT1 to modulate α-1,2-fucosylation and cancer-associated biology.

The Journal of biological chemistry·2026

Area of Science:

  • Glycomics and Glycobiology
  • Biotechnology and Bioengineering
  • Analytical Chemistry

Background:

  • Glycomics, the study of carbohydrates, faces challenges in high-throughput analysis compared to genomics and proteomics.
  • Current lectin microarrays rely on natural plant lectins with inconsistent activity, availability, and potential glycosylation issues.
  • A defined set of recombinant lectins is needed to improve reliability and accuracy in glycomic analysis.

Purpose of the Study:

  • To develop an efficient strategy for creating recombinant lectins for microarray technology.
  • To establish a reliable panel of bacterially-derived lectins with defined binding specificities.
  • To demonstrate the utility of a recombinant lectin microarray for distinguishing glycopatterns.

Main Methods:

More Related Videos

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Related Experiment Videos

Last Updated: Jul 5, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

  • Systematic creation and purification of bacterially-expressed recombinant lectins.
  • Characterization of lectin binding specificities using carbohydrate microarrays and ELISA.
  • Development and application of a recombinant lectin microarray for sample analysis.

    • Main Results:

    • A panel of simple-to-purify, bacterially-derived lectins with consistent activity was generated.
    • Lectin binding specificities were precisely defined.
    • The recombinant lectin microarray successfully distinguished glycopatterns in protein and cell samples.

    Conclusions:

    • Recombinant lectin production offers a reliable alternative to natural lectins for glycomic analysis.
    • This approach advances lectin microarray technology for high-throughput glycomics.
    • The systematic creation of defined lectins will enable broader applications in glycomic research.