Related Experiment Video
Updated: Jul 5, 2026
08:09
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Proteomics of marine bacteria
Thomas Schweder1, Stephanie Markert, Michael Hecker
1Institute of Marine Biotechnology, Greifswald, Germany. schweder@uni-greifswald.de
Electrophoresis
|May 22, 2008
Summary
Marine microorganisms
Area of Science:
- Marine microbiology
- Proteomics
- Genomics
Background:
- Limited understanding of marine microorganism life and adaptation strategies.
- Environmental challenges in marine habitats require specialized adaptations.
- Genomics and functional genomics offer new insights into these adaptations.
Purpose of the Study:
- To summarize current proteomic approaches for analyzing marine bacteria.
- To review state-of-the-art techniques in marine microbial proteomics.
- To discuss novel methods for metaproteome analysis of marine microbial consortia.
Main Methods:
- Proteome analysis of culturable, free-living marine bacteria.
- Proteomics applied to uncultivable symbiotic marine bacteria.
- Development of metaproteomic approaches for microbial consortia.
Main Results:
- Proteomics provides revolutionary insights into marine organism adaptation.
- Successful application of proteomics to diverse marine bacterial groups.
- Emerging techniques for analyzing complex microbial communities.
Conclusions:
- Proteomics is a powerful tool for understanding marine microbial life.
- Current methods enable analysis of both culturable and unculturable marine bacteria.
- Future research directions include metaproteomics for community-level insights.
More Related Videos
10:43Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
Published on: November 5, 2014
09:52A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
Published on: January 10, 2025
Related Concept Videos
01:18Deep Sea Microbial Ecology
The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches extending beyond...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
01:30Marine Microbial Ecology
Marine microbial ecosystems are shaped by distinct physicochemical limits, including high salinity, low nutrient availability, and fluctuating oxygen levels. These conditions favor smaller microbial cell sizes, which maximize their surface-to-volume ratio for efficient nutrient uptake.Microbial activity and community composition are closely linked to biogeochemical cycles, particularly in dynamic environments like estuaries, where halotolerant microbes thrive in response to variable salinity...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
01:26Bacterial Phylum Proteobacteria
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...