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The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva
Published on: May 16, 2012
Comparative analysis of three commercial saliva testing kits with a standard saliva buffering test
Y Kitasako1, M F Burrow, M Stacey
1Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. kitasako.ope@tmd.ac.jp
Background:
In 1959, Ericsson developed a laboratory buffer capacity test. Because the Ericsson test is not practical for use as a chair-side test, commercially available saliva buffering capacity tests have been developed for use in the dental office. The purpose of this study was to evaluate the correlation between a modified Ericsson test and three commercially available quantitative and colourimetric tests.
Methods:
Stimulated saliva (by chewing paraffin wax) was collected from 113 patients. Individual saliva buffering capacity was assessed with the following four different methods: modified Ericsson test; quantitative test using a hand-held pH meter; paper strip; or liquid colourimetric test. The correlations of ranking results among the different tests were analysed using the Spearman Rank Correlation Test, p < 0.001.
Results:
Spearman Rank Correlation indicated significant positive coefficients between the modified Ericsson test and the quantitative test (rho = 0.857), the paper strip colourimetric test (rho = 0.621) and the liquid-type colourimetric test (rho = 0.689).
Conclusion:
The detection level of medium and high buffering capacity was test dependent. The quantitative test using a hand-held pH meter showed a stronger positive correlation with the modified Ericsson test. The qualitative tests seemed less reliable, particularly for patients classified as having a medium buffering capacity.
