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Updated: Jul 5, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
[Effect of neoadjuvant chemotherapy on ERCC1 gene expression in breast cancer]
Jian-Min Fu1, Jie Zhou, Jian-Sheng Xie
1Department of Breast Diseases, Shenzhen Maternal and Child Health Hospital Affiliated to Southern Medical University, Shenzhen 518028, China. jmfu2000@yahoo.com.cn
Objective:
To investigate the expression of ERCC1 gene in breast cancer before and after neo-adjuvant chemotherapy.
Methods:
The expression of ERCC1 gene was detected by RT-PCR in 40 breast cancer patients and in 14 patients treated with neoadjuvant chemotherapy.
Results:
Positive expression of ERCC1 gene was detected by RT-PCR in 35.0% of the breast cancer specimens, and ERCC1 expression was not correlated to the patients' age, tumor size, axillary lymph node metastasis, pathological type, histological grade, ER, PR or HER-2 (P>0.05). ERCC1 gene expression was significantly higher in neo-adjuvant chemotherapy group than in non-chemotherapy group (P<0.05).
Conclusion:
The expression of ERCC1 gene does not affect the clinical and pathological features of breast cancer. Neo-adjuvant chemotherapy can increase the expression of ERCC1 gene, due attention should be given to with in subsequent chemotherapy.
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