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Updated: Jul 5, 2026

Mapping Infant Immunity with Minimal Input: Integrative Single-Cell and Multiomic Profiling
Published on: April 3, 2026
Postnatal maturation of total cell content and up-regulated surface expression of Mac-1 (CD11b/CD18) in
Shawn W Storm1, M Michele Mariscalco, Michael F Tosi
1Department of Pediatrics, Case Western University, Cleveland, Ohio, USA.
Abstract:
Markedly deficient expression of membrane-activated complex 1 (Mac-1; CD11b/CD18) by polymorphonuclear neutrophils (PMN) of human neonates compared with adults is well documented. To define postnatal maturation of Mac-1 expression of PMN, lysates of PMN from 21 infants, aged 1-14 months, and concurrent adult controls were assayed by ELISA for total cell content of Mac-1 and LFA-1 (CD11a/CD18), and LFA-1 content was within the normal adult range at all ages tested. Mac-1 content was approximately 50% of adult levels for infants 1-2 months of age and steadily increased to reach normal adult levels by 11-12 months of age. For a separate group of 25 infants, aged 0.5-11 months, measurement of surface expression of Mac-1 and LFA-1 on activated PMN by immunofluorescence flow cytometry yielded results that were similar to those obtained by ELISA.
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