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Atomic Fluorescence Spectroscopy

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

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Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

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Angular-Dependent Metal-Enhanced Fluorescence from Silver Island Films.

Kadir Aslan¹, Stuart N Malyn, Chris D Geddes

¹Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics and Laboratory for Advanced Fluorescence Spectroscopy, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard St., Baltimore, MD, 21201.

Chemical Physics Letters

|May 23, 2008

Summary

We observed angular-dependent Metal Enhanced Fluorescence (MEF) from fluorophores on silver island films (SiFs). This phenomenon showed a 10-fold enhancement, offering new insights into nanoscale optical properties.

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Area of Science:

Nanophotonics
Surface Science
Spectroscopy

Background:

Metal Enhanced Fluorescence (MEF) is a phenomenon where fluorophores near plasmonic nanostructures exhibit enhanced emission.
Silver island films (SiFs) are known for their plasmonic properties, making them suitable substrates for MEF studies.
Understanding MEF is crucial for developing advanced optical sensing and imaging technologies.

Purpose of the Study:

To investigate the angular dependence of Metal Enhanced Fluorescence (MEF) from fluorophores on silver island films (SiFs).
To quantify the MEF enhancement factor and its directional characteristics.
To assess the photostability of fluorophores under specific illumination and observation conditions.

Main Methods:

Fabrication of silver island films (SiFs) on glass substrates.
Immobilization of fluorescein isothiocyanate-human serum albumin (FITC-HSA) onto the SiFs.
Illumination of the samples with 473 nm laser light at incident angles of 45 and 90 degrees.
Measurement of scattered light and fluorescence emission at various observation angles.
Assessment of photostability by monitoring fluorescence intensity over time.

Main Results:

Observation of angular-dependent MEF from FITC-HSA immobilized on SiFs.
A significant 10-fold enhancement in MEF was observed.
The MEF signal showed a directional bias related to the incident light direction.
Scattering of incident laser light by SiFs was observed at wide angles.
Photostability of FITC was measured from the back of the substrate at specific angles.

Conclusions:

Angular-dependent MEF is a significant characteristic of fluorophores on SiFs.
The directional nature of MEF provides insights into the interaction between light, plasmonic nanostructures, and fluorophores.
SiFs offer a viable platform for studying and potentially enhancing fluorescence-based measurements.
The observed photostability is relevant for applications requiring long-term fluorescence detection.