Acceptance of routine ELISA testing among black women STD patients: relationship to patient-provider racial

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Published on: July 5, 2022

Chandra L Ford1, Thomas R Konrad, Dionne C Godette

  • 1Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY 10032, USA. cf2264@columbia.edu

Sexually Transmitted Diseases
|May 23, 2008
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.