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Related Concept Videos

Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Need for Obtaining Pure Cultures01:29

Need for Obtaining Pure Cultures

Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: Jul 5, 2026

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
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Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

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Natural product isolation.

Otto Sticher1

  • 1Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, Zurich 8093, Switzerland.

Natural Product Reports
|May 24, 2008
PubMed
Summary

Natural product research is advancing rapidly due to new separation and purification techniques. This review highlights modern methods for isolating novel chemical entities from natural sources, focusing on developments since 2000.

Area of Science:

  • Natural Product Chemistry
  • Analytical Chemistry
  • Separation Science

Background:

  • Interest in natural product research has surged since the 1990s.
  • Advances in separation methods, spectroscopy, and bioassays have revitalized the field.
  • Natural products are a key source of novel chemical entities.

Purpose of the Study:

  • To provide an updated review of natural product research.
  • To focus on sample preparation, purification, and extraction techniques.
  • To cover advancements in separation and isolation methods developed since 2000.

Main Methods:

  • Review of recent literature published since 2000.
  • Emphasis on modern extraction techniques.
  • Detailed discussion of liquid-solid and liquid-liquid isolation techniques.

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Last Updated: Jul 5, 2026

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
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Isolation of Soil Microorganisms Using iChip Technology
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Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
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Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

  • Analysis of multi-step chromatographic operations.
  • Main Results:

    • Significant progress in natural product separation and purification methods.
    • Development of novel extraction and isolation techniques.
    • Increased efficiency and sensitivity in analyzing natural products.

    Conclusions:

    • Modern separation techniques are crucial for discovering novel chemical entities from natural products.
    • The field continues to evolve with innovative approaches to sample preparation and purification.
    • Continued research is essential for unlocking the potential of natural product chemistry.