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Related Experiment Video

Updated: Jul 5, 2026

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Published on: August 9, 2017

The eye organizes neural crest cell migration.

Tobias Langenberg¹, Alon Kahana, Joseph A Wszalek

¹Department of Zoology, University of Wisconsin, Madison, Wisconsin 53706, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|May 24, 2008

Summary

Neural crest cells (NCCs) are crucial for eye development. This study reveals that the eyeless mutant chokh disrupts NCC migration, impacting eye development and potentially causing congenital diseases.

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Last Updated: Jul 5, 2026

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

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Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

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Published on: October 24, 2018

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

Area of Science:

Developmental Biology
Neuroscience
Genetics

Background:

Neural crest cells (NCCs) migrate to the anterior head, contributing to eye and orbit development.
Disruptions in NCC migration are linked to congenital eye diseases like glaucoma.
Mechanisms guiding anterior NCC migration to ocular tissues remain unclear.

Purpose of the Study:

To create a fate map of zebrafish diencephalic and mesencephalic NCC contributions to the eye and orbit.
To investigate the role of the eyeless mutant chokh/rx3 in anterior NCC guidance.

Main Methods:

Zebrafish fate mapping of diencephalic and mesencephalic NCCs.
Analysis of NCC migration in the eyeless mutant chokh/rx3.
Time-lapse microscopy to assess NCC migration rates and directionality.

Main Results:

Zebrafish NCC fate map shows evolutionary conservation with chick and mice.
Dorsal anterior NCC migration is severely disorganized in chokh mutants.
NCCs exhibit reduced migration rates and directionality in chokh mutants.

Conclusions:

The eyeless gene chokh plays a critical role in guiding anterior neural crest cell migration to the eye.
Understanding chokh's function offers insights into congenital eye disease mechanisms.
This study establishes a conserved role for chokh in vertebrate eye development.