Related Experiment Video
Updated: Jul 5, 2026
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Published on: August 29, 2018
Assignment and expression patterns of porcine muscle-specific isoform of phosphoglycerate mutase gene
Haifang Qiu1, Shuhong Zhao, Xuewen Xu
1Lab of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
Abstract:
It has been reported that the muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme; it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In this study, the cDNA of the porcine Pgam2 was cloned. This gene contains an open reading frame of 765 bp encoding a protein of 253 residues, and the predicted protein sequences share high similarity with other mammalians, 96% identity with humans, and 94% identity with mouse and rats. Pgam2 was mapped to SSC18q13-q21 by the RH panel. In this region, there are several QTLs, such as fat ratio, lean percentage, and diameter of muscle fiber, which affect meat production and quality. The reverse transcriptase-polymerase chain reaction revealed that the porcine Pgam2 gene was mainly expressed in the muscle tissue (skeletal muscle and cardiac muscle), and was expressed highly at skeletal muscle development stages (embryonic periods: 33, 65, and 90 days post-conception (dpc); postnatal pigs: 4 days and adult). This indicates that the Pgam2 gene plays an important role in muscle growth and development. In addition, it was demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei.
Insights
The porcine Pgam2 gene, crucial for energy release in glycolysis, is highly expressed in muscle tissues during development. This gene plays a significant role in muscle growth and meat quality traits.
Area of Science:
- Animal Genetics
- Molecular Biology
- Biochemistry
Background:
- Phosphoglycerate mutase (PGAM) is a housekeeping enzyme involved in glycolysis.
- The muscle-specific isoform, PGAM2, is encoded by the Pgam2 gene.
- Understanding Pgam2's role is vital for muscle development and meat production.
Purpose of the Study:
- To clone and characterize the porcine Pgam2 gene.
- To investigate the expression patterns of Pgam2 in porcine tissues.
- To determine the potential role of Pgam2 in muscle growth and meat quality.
Main Methods:
- Cloning of porcine Pgam2 cDNA.
- Sequence analysis and comparison with other mammalian species.
- Radiation hybrid (RH) mapping of Pgam2 to porcine chromosome 18.
- Reverse transcriptase-polymerase chain reaction (RT-PCR) for gene expression analysis.
Main Results:
- The porcine Pgam2 gene has an open reading frame of 765 bp, encoding 253 amino acids.
- Porcine Pgam2 protein sequences show high similarity (96%) to human and (94%) to mouse/rat sequences.
- Pgam2 was mapped to SSC18q13-q21, a region containing quantitative trait loci (QTLs) for meat production traits.
- Pgam2 is predominantly expressed in skeletal and cardiac muscle, with high expression during embryonic and postnatal muscle development.
- PGAM2 was found in both cytoplasm and nuclei, participating in glycometabolism.
Conclusions:
- The Pgam2 gene is conserved across mammals and plays a critical role in porcine muscle development.
- Its expression pattern suggests a significant involvement in muscle growth and potentially meat quality.
- The localization of PGAM2 in both cytoplasm and nuclei indicates diverse roles in cellular metabolism.
