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Sperm Structure and Semen Composition

Sperm Structure and Semen Composition

During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...

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Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

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Sperm parameters on Iberian red deer: electroejaculation and post-mortem collection.

A F Martínez¹, F Martínez-Pastor, M Alvarez

¹Animal Reproduction and Obstetrics, University of León, 24071 León, Spain.

|May 24, 2008

Summary

Sperm collection methods, post-mortem and electroejaculation, in Iberian red deer show potential for germplasm banking. Both methods yield usable sperm, with electroejaculation offering higher quality and lower abnormality rates for artificial reproductive technologies.

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Sperm Collection of Differential Quality Using Density Gradient Centrifugation

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Published on: November 29, 2018

Area of Science:

Veterinary Science
Reproductive Biology
Wildlife Conservation

Background:

Artificial reproductive technologies (ART) in cervids require foundational data for sperm collection.
Existing ART methods for cervids need further development and data collection.

Purpose of the Study:

To compare post-mortem (PM) and electroejaculation (EE) sperm collection models in Iberian red deer.
To analyze the relationship between stag age and sperm collection parameters.
To assess the suitability of collected sperm for germplasm banking and ART.

Main Methods:

Collected sperm from 179 wild red deer (PM) and 37 farmed red deer (EE).
Recorded testicular/epididymal weight, testicular diameter, sperm quantity, pH, osmolality, motility (CASA), abnormal forms, cytoplasmic droplets, viability, and acrosomal status.
Correlated parameters with stag age and compared PM vs. EE methods.

Main Results:

Genitalia parameters correlated linearly with stag age.
Sperm quantity was similar between PM (2.5x10^9) and EE (3.6x10^9), increasing with age only in PM.
EE samples showed significantly different pH and osmolality compared to PM, with higher motility quality, lower abnormal forms, and higher intact acrosomes (97% vs. 89%).

Conclusions:

Both post-mortem and electroejaculation sperm collection methods are viable for germplasm banking in Iberian red deer.
Electroejaculation yielded higher quality sperm with fewer abnormalities, making it potentially more advantageous for ART.
This study provides crucial spermatological data for advancing ART in cervids.