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Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
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Human bocavirus infection in children hospitalized with acute gastroenteritis in China

Jie-Mei Yu1, Dan-Di Li, Zi-Qian Xu

  • 1National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|May 24, 2008
PubMed

Insights

Human bocavirus (HBoV) is frequently found in children with gastroenteritis, often alongside other viruses. However, this study found no significant causal link between HBoV infection and gastroenteritis severity in children.

Area of Science:

  • Virology
  • Pediatrics
  • Gastroenterology

Background:

  • Human bocavirus (HBoV) initially identified in respiratory infections.
  • Increasing detection of HBoV in pediatric gastroenteritis fecal samples.

Purpose of the Study:

  • Determine HBoV prevalence in hospitalized children with gastroenteritis across China.
  • Investigate potential associations between HBoV and gastroenteritis.

Main Methods:

  • Analyzed 1216 fecal samples from children under 5 with acute gastroenteritis.
  • Utilized ELISA, RT-PCR, and PCR for pathogen detection.
  • Performed phylogenetic analysis of HBoV VP1/VP2 sequences using MEGA software.

Main Results:

  • HBoV detected in 67 specimens; 77.6% had co-infections with other common diarrheal viruses.
  • No statistically significant difference in clinical severity between rotavirus and HBoV co-infected children versus rotavirus-only infected children.
  • Phylogenetic analysis revealed a single genetic lineage of HBoV.

Conclusions:

  • HBoV is prevalent in pediatric gastroenteritis cases in China, frequently co-occurring with other viruses.
  • No statistically significant causal relationship established between HBoV and gastroenteritis in this pediatric cohort.
Abstract

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