Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AAV vector production in suspension cells using PEI transfection and sodium butyrate with orthogonal assessment of function and quality.

Molecular therapy. Advances·2026
Same author

Safety and immunogenicity of an investigational mRNA-lipid nanoparticle-based monovalent influenza vaccine: Results from a phase 1, randomized, dose-escalation study.

Human vaccines & immunotherapeutics·2026
Same author

PKP1 promotes lung cancer by modulating energy metabolism through stabilization of PFKP.

Biomarker research·2025
Same author

CXCR2 deficiency with myelokathexis caused by a novel variant: correction via CRISPR/Cas9.

Haematologica·2025
Same author

Gene editing in hematopoietic stem cells by co-delivery of Cas9/sgRNA ribonucleoprotein and templates for homology-directed repair in 'all-in-one' lentivirus-derived nanoparticles.

Nucleic acids research·2025
Same author

Targeted gene editing and near-universal cDNA insertion of CYBA and CYBB as a treatment for chronic granulomatous disease.

Nature communications·2025

Related Experiment Video

Updated: Jul 5, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Regulated gene insertion by steroid-induced PhiC31 integrase.

Nynne Sharma1, Brian Moldt, Trine Dalsgaard

  • 1Department of Human Genetics, University of Aarhus, DK-8000 Aarhus C, Denmark.

Nucleic Acids Research
|May 24, 2008
PubMed
Summary

Researchers developed inducible gene-inserting systems using hormone-binding domains. These systems allow precise control over transposase and recombinase activity, enhancing gene therapy safety and transgenesis applications.

More Related Videos

Embryo Microinjection for Transgenesis in Drosophila
05:32

Embryo Microinjection for Transgenesis in Drosophila

Published on: June 7, 2024

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
09:38

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Published on: February 2, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Embryo Microinjection for Transgenesis in Drosophila
05:32

Embryo Microinjection for Transgenesis in Drosophila

Published on: June 7, 2024

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
09:38

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Published on: February 2, 2021

Area of Science:

  • Molecular Biology
  • Genetic Engineering
  • Biotechnology

Background:

  • Nonviral integration systems are crucial genetic tools for transgenesis and gene therapy.
  • Efficient spatiotemporal regulation of gene-inserting proteins like transposases and recombinases is vital for their application.
  • Current methods for controlling these systems need improvement for enhanced safety and precision.

Purpose of the Study:

  • To investigate strategies for posttranslational induction of gene-inserting proteins.
  • To develop controllable transposase and recombinase systems for transgenesis and gene therapy.
  • To assess the efficacy and safety of engineered inducible systems.

Main Methods:

  • Fusion of engineered hormone-binding domains (hPR891, ER(T2)) with Sleeping Beauty, Frog Prince, piggyBac, Tol2 transposases, and Flp, PhiC31 recombinases.
  • Utilizing the synthetic steroid mifepristone for posttranslational induction.
  • Analyzing gene insertion inducibility in cultured human cells.

Main Results:

  • Demonstrated efficient posttranslational regulation of Flp recombinase and PhiC31 integrase activity using mifepristone.
  • Engineered PhiC31 integrase fused with ER(T2) showed a 22-fold induction and retained 75% of wild-type activity.
  • Successful mifepristone-directed inducibility of gene insertion was observed in human cells.

Conclusions:

  • Developed novel inducible PhiC31 integrase systems for precise control in transgenesis.
  • These inducible systems represent valuable tools for safety studies in gene therapy applications.
  • Posttranslational regulation offers a promising strategy for enhancing the utility of gene-inserting systems.