Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
07:52

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis.

Christine B Kost1, Jeannine T Holden, Karen P Mann

  • 1Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.

Cytometry. Part B, Clinical Cytometry
|May 27, 2008
PubMed
Summary

Flow cytometric immunophenotyping can distinguish marginal zone lymphoma (MZL) subtypes. CD11c expression identifies splenic MZL (SMZL), and CD19 levels help differentiate MZL from follicular lymphoma (FL).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodality Technologies in the Assessment of Hematolymphoid Neoplasms.

Archives of pathology & laboratory medicine·2017
Same author

Molecular Pathology of Malignant Lymphoma.

Surgical pathology clinics·2016
Same author

Clinical Validation and Implementation of a Targeted Next-Generation Sequencing Assay to Detect Somatic Variants in Non-Small Cell Lung, Melanoma, and Gastrointestinal Malignancies.

The Journal of molecular diagnostics : JMD·2016
Same author

Development and validation of CALR mutation testing for clinical diagnosis.

American journal of clinical pathology·2015
Same author

Clinical significance of quantitative monitoring and mutational analysis of BCR-ABL1 transcript in Philadelphia chromosome positive B lymphoblastic leukemia.

Leukemia & lymphoma·2015
Same author

Chromosome instability in diffuse large B cell lymphomas is suppressed by activation of the noncanonical NF-κB pathway.

International journal of cancer·2014

Area of Science:

  • Hematology
  • Oncology
  • Immunology

Background:

  • Marginal zone B-cell lymphoma (MZL) encompasses splenic (SMZL), nodal (NMZL), and extranodal MALT types.
  • Flow cytometric immunophenotyping (FCI) is crucial for diagnosing MZL, especially when morphology is insufficient.
  • Differentiating MZL from CD10-negative follicular lymphoma (FL) is a key diagnostic challenge.

Purpose of the Study:

  • To identify immunophenotypic markers specific to MZL and its subtypes using FCI.
  • To establish methods for distinguishing MZL from CD10-negative follicular lymphoma (FL).

Main Methods:

  • Retrospective review of FCI studies for 31 MZL cases and 31 FL cases.
  • Analysis of antigen expression patterns and intensity (mean channel fluorescence - MCF).

More Related Videos

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
09:02

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Published on: November 26, 2018

Related Experiment Videos

Last Updated: Jul 5, 2026

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
07:52

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
09:02

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Published on: November 26, 2018

  • Statistical comparison of antigen expression between MZL and FL groups.

    • Main Results:

    • All MZL and FL cases expressed CD19, CD20, and CD45.
    • CD11c expression was significantly higher in SMZL (57%) compared to other MZL subtypes (8%).
    • Brighter CD19 expression (mean MCF 455.3) was observed in MZL compared to FL (mean MCF 166.9).

    Conclusions:

    • MZL exhibits typical pan-B-cell antigen expression.
    • CD11c expression is a strong indicator for splenic MZL (SMZL).
    • Combined analysis of CD19, CD11c, and CD38 expression effectively differentiates MZL from CD10-negative FL.