Related Experiment Video
Updated: Jul 5, 2026
10:24
Quantitation of Endothelial Cell Adhesiveness In Vitro
Published on: June 18, 2015
Endothelial signalling by Ig-like cell adhesion molecules.
1Sanquin Research and Landsteiner Laboratory, Department Molecular Cell Biology, Academic Medical Center, University of Amsterdam, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands.
Summary
Leukocyte transendothelial migration involves signals from chemokines and cell adhesion molecules. Endothelial cells signal after leukocyte adhesion to aid migration across the cell layer.
Area of Science:
- Immunology
- Cell Biology
- Physiology
Background:
- Leukocyte transendothelial migration is crucial for immune responses.
- This process is regulated by complex signaling pathways involving both leukocytes and endothelial cells.
- Understanding these signals is key to modulating inflammatory processes.
Purpose of the Study:
- To provide an overview of leukocyte transendothelial migration.
- To highlight recent findings on adhesion-induced signaling in this process.
- To elucidate the role of endothelial signaling in facilitating leukocyte transmigration.
Main Methods:
- Literature review of leukocyte transendothelial migration.
- Analysis of chemokine-induced signaling pathways.
- Examination of integrin-ligand-induced bidirectional signaling.
- Discussion of endothelial signaling post-leukocyte adhesion.
Main Results:
- Leukocyte migration is controlled by chemokine signaling in leukocytes.
- Bidirectional signaling between leukocytes and endothelial cells via integrin-ligands is critical.
- Endothelial cells generate signals after leukocyte adhesion to promote transmigration.
- Both paracellular and transcellular routes of migration are influenced by endothelial signaling.
Conclusions:
- Endothelial signaling plays a vital role in facilitating leukocyte transendothelial migration.
- Recent research provides new insights into adhesion-induced signaling mechanisms.
- Further understanding of these pathways can inform therapeutic strategies for inflammatory diseases.
Related Concept Videos
01:31Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
01:17Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
01:25Selectins
Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
01:20Cell Adhesion Molecules - Types and Functions
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved in a...
CAM Families
The Integrin family of proteins is primarily involved in a...
01:20Cell Adhesion Molecules - Types and Functions
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved in a...
CAM Families
The Integrin family of proteins is primarily involved in a...
01:24Adherens Junctions
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Adherens Junctions are Dynamic
The endothelial cells...