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Updated: Jan 10, 2026

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
Published on: April 10, 2020
Multiple roles for protein palmitoylation in plants
Piers A Hemsley1, Claire S Grierson
1School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK. piers.hemsley@bristol.ac.uk
Abstract:
Palmitoylation, more correctly known as S-acylation, aids in the regulation of cellular functions including stress response, disease resistance, hormone signalling, cell polarisation, cell expansion and cytoskeletal organization. S-acylation is the reversible addition of fatty acids to proteins, which increases their membrane affinity. Membrane-protein interactions are important for signalling complex formation and signal propagation, protein sequestration and segregation, protein stability, and maintaining polarity within the cell. S-acylation is a dynamic modification that modulates the activity and membrane association of many signalling molecules, including ROP GTPases, heterotrimeric G-proteins and calcium-sensing kinases. Recent advances in methods to study S-acylation are permitting an in-depth examination of its function in plants.
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