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Detection of BVD viruses using synthetic oligonucleotides
T L Lewis1, J F Ridpath, S R Bolin
1Department of Veterinary Science, North Dakota State University, Fargo.
Archives of Virology
|January 1, 1991
Summary
Synthetic oligonucleotide probes show promise for diagnosing bovine viral diarrhea virus (BVDV). One probe detected 86% of BVDV isolates, demonstrating potential for rapid and specific viral detection in cattle.
Area of Science:
- Veterinary Virology
- Molecular Diagnostics
- Oligonucleotide Technology
Background:
- Bovine viral diarrhea virus (BVDV) poses a significant threat to cattle health and the agricultural economy.
- Accurate and rapid diagnostic tools are crucial for controlling BVDV outbreaks.
- Synthetic oligonucleotide probes offer a potential avenue for specific viral detection.
Purpose of the Study:
- To evaluate the efficacy of synthetic oligonucleotide probes for the detection of BVDV.
- To identify optimal probe sequences for broad BVDV isolate detection.
- To assess the utility of these probes for direct detection in infected cattle tissues.
Main Methods:
- Homology analysis of BVDV genomic sequences to select six 20-base oligonucleotide probes.
- RNA extraction from 22 BVDV isolates propagated in bovine turbinate cells.
- Hybridization assays using 32P end-labeled probes under stringent conditions to ensure specificity.
Main Results:
- One probe (ND001) detected 86% of BVDV isolates, while others ranged from 19% to 57% detection.
- Probes successfully detected both cytopathic and noncytopathic BVDV isolates.
- A probe cocktail enabled specific detection of BVDV RNA directly from infected cattle tissues.
Conclusions:
- Synthetic oligonucleotide probes are effective diagnostic tools for BVDV detection.
- The ND001 probe demonstrates high sensitivity for a broad range of BVDV isolates.
- These probes facilitate specific detection of BVDV in clinical samples, aiding in disease management.