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Subcellular distribution of Cd and Pb in earthworm Eisenia fetida as affected by Ca2+ ions and Cd-Pb interaction
Lian-Zhen Li1, Dong-Mei Zhou, Peng Wang
1State Key Laboratory of Soil and Sustainable Agriculture, Department of Soil Chemistry and Environmental Protection, The Institute of Soil Science, Chinese Academy of Sciences, No. 71, East Beijing Road, Nanjing 210008, China. lzli@issas.ac.cn
Abstract:
A semi-static solution culture method was used to study the effects of Ca(2+) supply and interaction of Cd-Pb on the subcellular distribution of Cd and Pb in earthworm Eisenia fetida. The subcellular distribution of Cd and Pb was shown to be metal specific. About 80% of the Cd was distributed in the cytosol (fraction G), and only about 20% of total Cd was found in the tissue and cell membrane (fraction E) and the microsomes (fraction F). Nearly 50% of the Pb was rich in the tissue and cell membrane (fraction E). The supply of Ca(2+) ions significantly decreased Cd concentration in the cytosol (fraction G) and the whole tissue of the E. fetida. At the subcellular level, the addition of Pb(2+) ions significantly decreased the Cd percentage associated with fraction G from 83.7% to 58.4% and increased fraction E from 10.7% to 34.0%, respectively.
