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Updated: Jul 5, 2026

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
Published on: November 8, 2024
Effects of processing barley on its digestion by horses
I Vervuert1, K Voigt, T Hollands
1Institute of Animal Nutrition, Nutritional Diseases and Dietetics, Faculty of Veterinary Medicine, University of Leipzig, Gustav-Kührstrasse 8, D-04159 Leipzig, Germany.
Different barley processing methods significantly impact horse digestion. Extruded barley led to higher glycemic and insulin responses, indicating less pre-cecal starch digestion compared to rolled or micronized barley.
Area of Science:
- Equine nutrition
- Digestive physiology
Background:
- Starch digestibility varies with barley processing.
- Understanding equine starch digestion is crucial for diet formulation.
Purpose of the Study:
- To evaluate the impact of rolled, micronized, and extruded barley on equine starch digestion.
- To assess pre-cecal starch digestibility and hindgut fermentation in horses fed different barley preparations.
Main Methods:
- Four horses were fed diets with rolled, micronized, or extruded barley (2 g starch/kg bodyweight).
- Blood samples measured glycemic and insulinemic responses.
- Breath samples analyzed for hydrogen and methane to assess fermentation.
Main Results:
- Extruded barley elicited the highest peak glucose and insulin levels, suggesting lower pre-cecal starch digestibility.
- Micronized barley resulted in intermediate responses.
- Rolled barley showed the lowest glycemic and insulinemic responses.
- Postprandial breath hydrogen and methane levels varied, with no significant differences between treatments, but a trend towards higher methane with rolled barley.
Conclusions:
- Barley processing significantly influences starch digestion and absorption in horses.
- Extrusion appears to reduce pre-cecal starch digestibility the most among the tested methods.
- Further research is needed to fully elucidate the implications of these findings on hindgut fermentation and overall equine health.
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